Staphylococcus can be identified using mannitol salt agar, and this agar's high salt concentration favors those bacteria. Mannitol salt agar is also selective for those bacteria.
Mannitol salt agar
- Mannitol, a sugar alcohol, and phenol red, a PH indicator are also present.
- Red phenol Normally it has a neutral pH, however mannitol fermentation will cause it to turn yellow at an acid pH.
- Mannitol and sodium chloride (7.5%) are both present in mannitol salt agar (MSA).
- The pH indicator, phenol red, which is pinkish-red at neutral pH but yellow under acidic circumstances, is also present in the medium.
- Bacteria that can tolerate a lot of salt, like those on the skin, can grow on this medium, notably Staphylococcus species.
- Acid is created as a byproduct of fermentation when mannitol is fermented by the bacteria growing on the agar, which causes the media surrounding the colonies to appear yellow.
- Around bacteria that do not ferment mannitol, no change in color is seen.
- Staphylococcus aureus growth on MSA as a mannitol fermenter has been utilized as a preliminary indication.
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