Chromatography is primarily used as a method for the analysis and separation of chemical mixtures. Chromatography is the process of causing the constituent parts of a mixture to migrate differently from a restricted starting point in a particular medium, in this case, filter paper.
A) The air pressure above the beaker would disrupt the flow of the spots through the TLC, resulting in less resolution on the TLC plate, if the watch glass is not kept above the beaker for running paper chromatography. Despots would look, and even swarm, as a result.
B) The solvent is not allowed to move completely through the TLC plate because if the chromatogram is left in the beaker for a long time so that the solvent completely flows to the top, all of the spots would reach the solvent front and we would be unable to distinguish between the spots present in the TLC plate.
C). The TLC spotting should be performed in a very minute manner. Assume that if this substance is quite thick and a few of spots are seen apart, the TLC plate would drag and the resolution would be thrown off. As a result, it is challenging to tell the spots apart.
D). If the chromatographic paper is very short, it will be difficult to distinguish between the spots because, if more than one spot is present in a given compound, it will be harder to determine how many spots there are if there is a smaller distance between them. The TLC sheet's shorter length is the cause of the decreased space between the supports. Therefore, it is preferable to avoid using this longer TLC sheet so that the resolution is high and the spots can be easily identified.
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