DNA ligase is used to join Okazaki fragments after the removal of RNA primer. However, DNA synthesis should occur in both directions since the double-stranded DNA is antiparallel. Therefore, Okazaki fragments are created during the lagging template strand synthesis.
The majority of organisms' genetic material is encoded in DNA. In DNA, two antiparallel DNA strands are linked together by hydrogen bonds to form a double-stranded molecule. The entire genome should be copied during cell division, doubling the amount of DNA in the parent cell. One of the DNA strands in the freshly formed double-stranded DNA is an original strand, and DNA replication proceeds in a semi-conservative way. Therefore, DNA replication should use both strands as a template. The enzyme in charge of DNA replication is called DNA polymerase. Only DNA in the 5' to 3' orientation is synthesized by it. However, DNA synthesis should occur in both directions since the double-stranded DNA is antiparallel. Therefore, Okazaki fragments are created during the lagging template strand synthesis.
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