A special enzyme called DNA primase uses the unwound DNA strand as a template to make it easier to synthesize an RNA primer.
The process uses an enzyme called DNA primase to create short RNA primers on the lagging strand using ribonucleoside triphosphates. These primers, which are generated in eucaryotes at intervals of 100–200 nucleotides on the lagging strand, are around 10 nucleotides long. RNA primers, oligonucleotides that are complementarily attached to a nucleic acid polymer, are created by the enzyme primase. Primase is necessary because DNA polymerases can only extend from the 3′-hydroxyl of a primer and cannot begin polymer synthesis on single-stranded DNA templates.
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