Polymerase activity ability to nick intact is - 5' to 3' Exonuclease.
An enzyme called DNA polymerase I takes role in the replication of bacterial DNA. It was the first known DNA polymerase, and Arthur Kornberg discovered it in 1956. It was first identified in E. coli and is found in all prokaryotes.
Activities ; In addition to having a 3'–5' exonuclease activity, or "proofreading" function, DNA Polymerase I also has a 5'–3' exonuclease activity, which allows the enzyme to replace nucleotides in the expanding strand of DNA via nick translation.
Importance ; By eliminating RNA primers and substituting ribonucleotides with DNA, Pol I helps join Okazaki fragments as part of its physiological role in supporting the repair of damaged DNA.
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