What is an advantage of a culture-based pathogen identification protocol over a culture-independent identification protocol?

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Because of their practical benefits, culture-independent methods for characterizing microbial communities have grown in popularity in recent years.

What is identification protocol?

  • Because of their practical benefits, culture-independent methods for characterizing microbial communities have grown in popularity in recent years.
  • The primary advantage of culture-independent methods is their ability to quickly identify a large proportion of the bacterial diversity that culture-based studies can make difficult to observe.
  • Although the current culture-based methods are limited in terms of the types of organisms detected, they have the advantage of quantifying absolute cell abundances of culturable living microbes.
  • Bacterial diversity reveals details about the evolution and mechanisms of life. Using DNA or rRNA sequence data, it is possible to determine the evolutionary relationships between various creatures.
  • These connections could deviate from traditional taxonomic hierarchies.

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