Reasons why you need to cut the plasmids before inserting them into the fertilized egg, is that the plasmids may or may not help the conjugation.
Plasmids
Plasmids can be defined as small circular DNA molecules that are found in bacteria and also in yeast. Plasmids have genetic information that is not extremely essential for the survival of the bacterium, but is usually related to some adaptive function for special situations.
Classification of plasmids
Plasmids can be classified in different ways, making it possible to identify, for example, two basic groups.
- Conjugatives: These plasmids ensure the initiation of conjugation, a process in which genetic material is exchanged between bacteria.
- Non-conjugative: These plasmids do not allow initiation of conjugation.
With this information, we can say that plasmids can help conjugation or not allow conjugation, linked to bacterial reproduction.
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