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The anticoagulant in a gray-topped tube destroys many enzymes when collecting specimens for ALT or AST. This grey-top tube contains potassium oxalate and sodium fluoride.

The gray-topped tube has potassium oxalate which acts as an anticoagulant that can destroy different enzymes, it is used for the determination of glucose in a plasma sample.

This tube also contains sodium fluoride, which enables the preservation of glucose in the whole blood during specific tests.

Sodium fluoride can inhibit the activity of enolase, which is a key enzyme involved in the last stages of glycolysis.

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