A reliable DNA analysis is based on the DNA conditions. The importance of using undigested cells is that DNA is well-preserved, not affected by external factors, and can be used to compare it to database sequences.
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Dr. Pringle studies niche partitioning and competition reduction among coexistent species in Africa.
He is interested in knowing the exact source of food of different herbivorous species. To do so, he is using the technique of DNA metabarcoding.
He collects fresh animals' dung and gets the indigested plant cells.
DNA is isolated, sequenced, and compared with the DNI of known species, which are a potential source of food for these animals.
Once he matches the cells' DNA with the corresponding plant species from the database, he can use this information to detail the animal's source of food.
The importance of getting fresh, undigested cells from the dung, is that
- The animal's digestive enzymes have not broken the cell walls and digested the cell content.
- The dung environmental conditions such as pH, temperature, or microbiota have not affected the cell's DNA.
- Undigested cells' DNA is well-preserved and can be used to compare it with the plant species database.
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