In an experiment, a scientist isolates mitochondria from living cells and suspends them in two different buffered solutions. One solution is maintained at pH 4, while the other solution is maintained at pH 9. The
scientist finds that mitochondria in the solution at pH 4 continue to produce ATP but those in the pH 9 solution do not
The results of the experiment can be used as evidence in support of which of the following scientific claims about mitochondrial activity?
Mitochondria in a cell free environment are unable to convert thermal energy into ATP.
The electron transport chain pumps electrons from the cytosol to the mitochondrial matrix,
CATP production in mitochondria requires a hydrogen ſon gradient that favors movement of protons into the mitochondrial matrix.
D
ATP synthase molecules change their orientation in relation to the proton gradient across the mitochondrial membrane.

The scientific claim 'ATP production in mitochondria requires a hydrogen ion gradient that favors the movement of protons into the mitochondrial matrix' SUPPORTS mitochondrial activity (Option C).

Cellular respiration is defined as a group of metabolic reactions by which cells can generate ATP (the energy coin of the cell), by using oxygen and the energy released in the chemical bonds of foods (e.g., glucose).

Cellular respiration has three main types of reactions: glycolysis, the acid citric cycle and oxidative phosphorylation.

Glycolysis occurs in the cytoplasm, whereas the citric acid cycle and oxidative phosphorylation occur in the mitochondria.

During oxidative phosphorylation, the movement of electrons through a series of carriers is coupled to the generation of an electrochemical proton (H+) gradient across the inner mitochondrial membrane.

Subsequently, this proton (H+) gradient is then used to generate ATP by using an ATP synthase.

In conclusion, the scientific claim 'ATP production in mitochondria requires a hydrogen ion gradient that favors the movement of protons into the mitochondrial matrix' SUPPORTS mitochondrial activity (Option C).

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