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The scientist anneals a mutant oligonucleotide to the original M13 DNA. Then DNA polymerase replicates the M13 DNA. After DNA synthesis, the scientist adds a restriction enzyme, EcoRI, that recognizes the DNA sequence 5′−GAATTC−3′5′−GAATTC−3′ and cuts after the guanosine nucleotide. Following the restriction digest, the scientist adds exonuclease III for a short period of time. Another round of DNA polymerase‑catalyzed DNA synthesis completes the procedure. What is the nucleotide mixture required by the DNA polymerase reaction in this procedure?

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annyksl

Answer:

The mixture of nucleotides required for this procedure is called dNTPs.

Explanation:

When the polymerase is used in a PCR procedure, to replicate a molecule or DNA fragment, use a combination of nucleotides called dNTPs.

DNTPs are nothing less than DNA nucleotides, applied as reagents in a PCR solution, which provide the material necessary for the polymerase to replicate DNA, even outside a biological system.

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