Explanation:
1. Unwinds DNA molecule- DNA helicase
During DNA replication, DNA helicases performs the function of unwinding the DNA at origin of replication where synthesis will be initiated and it continues to unwind the DNA forming replication fork.
hence, DNA helicases unwinds DNA molecule.
2.synthesizes the complementary DNA strands- DNA polymerase
During DNA replication, enzyme performs the function of adding nucleotides (3')-end of a DNA strand of DNA.
hence, DNA polymerase synthesizes the complementary DNA strands.
3.synthesizes RNA primer at the initiation point- RNA primase
During DNA replication, RNA primase synthesizes a primer for replication to start as DNA polymerase binds to the primer.
hence, RNA primase synthesizes RNA primer.
4.creates nick ahead of the replication fork to relieve tension from unwinding- DNA topoisomerase
During DNA replication, DNA Topoisomerase make single-stranded breaks in DNA to allow this process to proceed as the replication machinery binds to unwound DNA to replicate.
hence, DNA topoisomerase creates nick ahead of the replication fork to relieve tension from unwinding.
5.joins the okazaki fragmets together- DNA ligase
During DNA replication, DNA ligase enzyme bonds single-strand break in DNA by forming a phosphodiester bond between 5′-monophosphate and 3′-OH group.
hence, DNA ligase joins the okazaki fragmets together.
