Which of the following events does not take place during pyruvate oxidation?
A. Pyruvate is oxidized to form acetyl CoA.
B. Acetyl CoA formation occurs in the mitochondrial matrix in eukaryotes.
C. Cytoplasmic NAD+ is reduced in eukaryotes.
D. One molecule of CO₂ is produced for each pyruvate oxidized.
E. Coenzyme A binds to the acetate molecule.