As part of your research project, you are characterizing the 5' regulatory region of a gene. Specifically, you want to determine the region necessary for expression. You make successive deletions from the 5' end of the gene and assay for transcriptional activity by measuring production of an enzyme.
Consider the following experimental results below. The sequences given refer to base pairs and are relative to the start site of transcription.

Sequencetested Transcriptional activity
Control -150 to +50 100%
Sample 1 -120 to +50 66%
Sample 2 -90 to +50 63%
Sample 3 -60 to +50 67%
Sample 4 -30 to +50 67%
Sample 5 0 to +50 0%

The results suggest that regulatory region(s) lie
A. between -150 to -120 and between -30 to 0.
B. between -30 and 0 only.
C. between -120 and -30.
D. between -120 to -90, between -30 to 0.